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Promega™ pGL4 Luciferase Reporter Vectors


Available in numerous configurations for enhanced usability and convenience

Manufacturer: promega™  E6771

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Catalog No. PRE6771

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Description & Specifications

Specifications

Concentration 1μg/μL
For Use With (Application) Transcription regulation, virus-cell interactions, compound screening, post-translational modifications, GPCR signaling, cell signaling, promoter analysis
Marker Gene Puro
Primer RV primer 3 and RV primer 4
Protein Degradation Sequence hCL1-hPEST
Reporter Gene luc2A
Quantity 20μg
Vector pGL4.22[luc2CP/Puro] vector
Storage Requirements -20°C
Storage Buffer 10mM Tris HCl (pH 7.4), 1mM EDTA

  • Configurations available with the synthetic firefly luc2 (Photinus pyralis) and Renilla hRluc (Renilla reniformis) genes, which have been codon optimized for more efficient expression in mammalian cells
  • Both the reporter genes and the vector backbone, including the bla (β-lactamase or Ampr) and mammalian selectable marker genes for hygromycin (Hygro or Hygr), neomycin (Neo or Neor) and puromycin (Puro or Puror), have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription
  • The backbone is also supplied with two Rapid Response™ Luciferase Reporter genes (-P, -CP) for each luciferase gene; the proteins encoded by these Rapid Response genes respond more quickly and with greater magnitude to changes in transcriptional activity than their more stable counterparts
  • Choice of mammalian selectable markers provides easy transition from transient to stable cells
  • Use of a common multiple cloning site and a unique Sfil transfer scheme permits easy transfer from vector to vector

Vector Options

  • Basic vectors with no promoter that contain a multiple cloning region for cloning a promoter of choice
  • Vectors containing a minimal promoter
  • Vectors containing response elements (cAMP, NFAT, NF-κB, GAL4UAS) and a minimal promoter
  • Promoter-containing vectors that can be used as expression controls or as coreporter vectors

Transcription regulation, Virus-cell interactions, Compound screening, Post-translational modifications, GPCR signaling, Cell signaling, Promoter analysis