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Promega™ pGL3 Luciferase Reporter Vectors

Offer increased expression and a modified coding region for firefly luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells

Manufacturer: promega™  E1771

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Catalog No. PR-E1771

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Description & Specifications


For Use With (Application) Quantitative analysis of factors that potentially regulate mammalian gene expression
Includes 20μg Vector DNA, 500μL JM109 bacterial strain
Promoter SV40
Reporter Gene Luciferase
Quantity 20μg
Vector pGL3-enhancer vector
Storage Requirements Vector at -20°C and bacterial strain at -70°C

  • The backbone of the pGL2 Luciferase Reporter Vectors was redesigned for the pGL3 Vectors
  • pGL3 Vectors provide a basis for rapid, sensitive, and quantitative analysis of factors that potentially regulate mammalian gene expression
  • Nco I site located at 5« end of the luc+ gene allows creation of fusions with the reporter gene using a unique Nco I site
  • Placement of a Sma I site in the multiple cloning site (MCS) allows blunt-ended inserts to be ligated into the MCS and restricted on either side by other restriction endonucleases
  • Creation of an Xba I site just downstream of the luc+ gene facilitates insertions into the 3« untranslated region of mRNA or subcloning of the luciferase gene

The specific transcriptional characteristics of the pGL3 Vectors will vary for different cell types, particularly COS cells