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Promega™ pGL3 Luciferase Reporter Vectors

Offer increased expression and a modified coding region for firefly luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells

$485.78 - $485.78

Specifications

For Use With (Application) Quantitative analysis of factors that potentially regulate mammalian gene expression
Includes 20μg Vector DNA, 500μL JM109 bacterial strain
Quantity 20μg
Reporter Gene Luciferase
Storage Requirements Vector at -20°C and bacterial strain at -70°C
View More Specs
Includes:  Vector DNA, 20μg; Bacterial strain JM109, 500μL
Products
Catalog Number Mfr. No. Vector Price Quantity    

PR-E1741

 
promega™
E1741
pGL3-control vector Each for $485.78

PR-E1761

 
promega™
E1761
pGL3-promoter vector Each for $485.78

PR-E1751

 
promega™
E1751
pGL3-basic vector Each for $485.78

PR-E1771

 
promega™
E1771
pGL3-enhancer vector Each for $485.78
Description & Specifications

Specifications

For Use With (Application) Quantitative analysis of factors that potentially regulate mammalian gene expression
Includes 20μg Vector DNA, 500μL JM109 bacterial strain
Quantity 20μg
Reporter Gene Luciferase
Storage Requirements Vector at -20°C and bacterial strain at -70°C

  • The backbone of the pGL2 Luciferase Reporter Vectors was redesigned for the pGL3 Vectors
  • pGL3 Vectors provide a basis for rapid, sensitive, and quantitative analysis of factors that potentially regulate mammalian gene expression
  • Nco I site located at 5« end of the luc+ gene allows creation of fusions with the reporter gene using a unique Nco I site
  • Placement of a Sma I site in the multiple cloning site (MCS) allows blunt-ended inserts to be ligated into the MCS and restricted on either side by other restriction endonucleases
  • Creation of an Xba I site just downstream of the luc+ gene facilitates insertions into the 3« untranslated region of mRNA or subcloning of the luciferase gene

The specific transcriptional characteristics of the pGL3 Vectors will vary for different cell types, particularly COS cells

1U.S. Patent 5,670,356.
2The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.