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Promega™ pGEM™ Vectors


Enable blue/white screening and contain both the SP6 and T7 RNA polymerase promoters

Manufacturer: promega™  P2271

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Catalog No. PR-P2271

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Description & Specifications

Specifications

For Use With (Application) Synthesis of RNA in-vitro and allows selection of recombinants by blue/white screening
Includes 500μL Glycerol stock of bacterial strain JM109
Promoter Sp6 and T7
Restriction Site EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII
Vector pGEM-3Zf(+) vector
Storage Requirements Vector at -20°C and bacterial strain at -70°C

 

  • Multiple cloning site provides a selection of restriction sites and is flanked by the SP6 and T7 RNA polymerase promoters
  • lacZ α-peptide and multiple cloning site arrangement from pUC18 allow recombinants to be selected using blue/white screening
pGEM-3Z and -4Z Vectors†
  • Versatile vectors can be used for standard cloning and in vitro transcription
  • pGEM-3Z and pGEM-4Z vectors are identical except for the orientation of the SP6 and T7 promoters
pGEM-3Zf(+/-), -5Zf(+/-), -7Zf(+/-), -9Zf(-), -11Zf(+/-), and -13Zf(+) Vectors†
  • Vectors are derived from the pGEM-3Z vector and contain the origin of replication of the filamentous phage f1
  • Versatile vectors can be used for standard cloning, ssDNA production, and in vitro transcription
  • (+) and (-) vectors with the same numeric designation are identical except for the orientation of the f1 origin
  • pGEM-5Zf(+/-) and -7Zf(+/-) contain restriction site arrangements designed to generate unidirectional deletions with the Erase-a-Base™ System
  • pGEM-9Zf(-) permits the excision of an insert containing the SP6 and T7 polymerase promoters