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Promega™ pGEM™ Vectors DFS Item

Enable blue/white screening and contain both the SP6 and T7 RNA polymerase promoters

Manufacturer:  Promega™ P2411

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Catalog No. PR-P2411



Description

Description

 

  • Multiple cloning site provides a selection of restriction sites and is flanked by the SP6 and T7 RNA polymerase promoters
  • lacZ α-peptide and multiple cloning site arrangement from pUC18 allow recombinants to be selected using blue/white screening
pGEM-3Z and -4Z Vectors†
  • Versatile vectors can be used for standard cloning and in vitro transcription
  • pGEM-3Z and pGEM-4Z vectors are identical except for the orientation of the SP6 and T7 promoters
pGEM-3Zf(+/-), -5Zf(+/-), -7Zf(+/-), -9Zf(-), -11Zf(+/-), and -13Zf(+) Vectors†
  • Vectors are derived from the pGEM-3Z vector and contain the origin of replication of the filamentous phage f1
  • Versatile vectors can be used for standard cloning, ssDNA production, and in vitro transcription
  • (+) and (-) vectors with the same numeric designation are identical except for the orientation of the f1 origin
  • pGEM-5Zf(+/-) and -7Zf(+/-) contain restriction site arrangements designed to generate unidirectional deletions with the Erase-a-Base™ System
  • pGEM-9Zf(-) permits the excision of an insert containing the SP6 and T7 polymerase promoters

 

Specifications

Specifications

Synthesis of RNA in-vitro and allows selection of recombinants by blue/white screening
Sp6 and T7
20μg
−30 to −10°C
500μL Glycerol stock of bacterial strain JM109
SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII
pGEM-11Zf(+) vector
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