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Promega™ pGEM™-T and pGEM™-T Easy Vector Systems DFS Item

Convenient systems for the routine subcloning of PCR products

Manufacturer:  Promega™ A1380

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Catalog No. PR-A1380


 

Specifications Description & Specifications

Specifications

For the routine subcloning of PCR products
Sp6 and T7
20 Reactions
Store competent cells at -70°C, store all other components at -20°C
1.2μg pGEM-T easy vector (50ng/μL), 12μL control insert DNA (4ng/μL), 200μL rapid ligation Buffer (2X), 100U T4 DNA Ligase, JM109 1.2mL competent cells high efficiency (6 x 200μL)
BstZI, NotI and EcoRI
pGEM-T easy vector II