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Promega™ pGEM™-T and pGEM™-T Easy Vector Systems

Convenient systems for the routine subcloning of PCR products

Manufacturer: promega™  A1360

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Catalog No. PR-A1360


Description & Specifications


For Use With (Application) For the routine subcloning of PCR products
Includes 1.2μg pGEM-T easy vector (50ng/μL), 12μL control insert DNA (4ng/μL), 200μL rapid ligation Buffer (2X), 100U T4 DNA Ligase
Promoter Sp6 and T7
Restriction Site BstZI, NotI and EcoRI
Quantity 20 Reactions
Vector pGEM-T easy vector I
Storage Requirements -20°C

The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with EcoR V and adding a 3« terminal thymidine to both ends. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid.

  • 2X Rapid Ligation Buffer allows reactions to be completed in one hour at room temperature Blue/white screening can be used to directly identify recombinant clones, as T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase, permitting insertional inactivation of the α-peptide
  • f1 Origin of Replication allows the preparation of single-stranded DNA

pGEM-T Vector Systems

  • Multiple cloning site is flanked by recognition sites for the restriction enzyme BstZ I Single-enzyme digestion allows release of the insert
  • Double digestion may also be used to release the insert from the vector
pGEM-T Easy Vector Systems
  • Recognition sites for BstZ I, EcoR I, and Not I flank the insertion site Several options are available for removal of the desired insert DNA with a single restriction digestion