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Promega™ PepTag™ Nonradioactive Protein Kinase Assays DFS Item

The PepTag Non-Radioactive Protein Kinase Assay Systems provide a rapid, sensitive, non-radioactive method to detect either Protein Kinase C (PKC) or Protein Kinase A (PKA) activity

Manufacturer:  Promega™ V5340

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Catalog No. PR-V5340



Includes: Purified kinase as a positive control

Description

Description

  • Use brightly colored fluorescent peptide substrates that are highly specific for PKC and PKA
  • Detect less than 2ng of purified kinase in under two hours
  • Can detect kinase activity in partially purified samples as well as in purified preparations of enzymes
  • Low background
  • Quantitation of phosphorylated peptide can be accomplished using a densitometer, spectrophotometer, 96-well plate reader or fluorometer

U.S. Patents 5,580,747 and other patents pending.

Specifications

Specifications

Non radioactive cAMP-dependent assay
Uses brightly colored Fluorescent Peptide Substrates to detect less than 2ng of purified Kinase in under two Hours
<−65°C
120 Reactions
Purified Kinase as a positive control, 650μL PepTag™ PKA Reaction 5X Buffer, 650μL PKA Activato r 5X Solution, 25μL cAMP-dependent Protein Kinase, Catalytic Su
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