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Promega™ PepTag™ Nonradioactive Protein Kinase Assays

Rapid, sensitive screening of column fractions or for kinase activators and inhibitors

Manufacturer: promega™  V5340

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Catalog No. PR-V5340


Description & Specifications


Product Type Non radioactive cAMP-dependent assay
Quantity 120 Reactions
For Use With (Application) Uses brightly colored Fluorescent Peptide Substrates to detect less than 2ng of purified Kinase in under two Hours
Includes Purified Kinase as a positive control, 650μL PepTag™ PKA Reaction 5X Buffer, 650μL PKA Activato r 5X Solution, 25μL cAMP-dependent Protein Kinase, Catalytic Su
Storage Requirements -70°C

  • Use brightly colored fluorescent peptide substrates that are highly specific for PKC and PKA
  • Detect less than 2ng of purified kinase in under two hours
  • Can detect kinase activity in partially purified samples as well as in purified preparations of enzymes
  • Low background
  • Quantitation of phosphorylated peptide can be accomplished using a densitometer, spectrophotometer, 96-well plate reader or fluorometer