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Promega™ pCI and pCI-neo Mammalian Expression Vectors


Versatile vectors with multiple cloning sites

Manufacturer: promega™  E1731

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Catalog No. PR-E1731

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Description & Specifications

Specifications

For Use With (Application) Promote constitutive expression of cloned DNA inserts in Mammalian cells, can also be used to synthesize transcripts in-vitro using the T7 RNA Polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication
Promoter CMV
Quantity 20μg
Vector pCI vector
Storage Requirements -20°C
Storage Buffer TE Buffer (pH 8)

pCI Mammalian Expression Vector

  • Designed to promote constitutive expression of cloned DNA inserts in mammalian cells
  • Contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region†
  • Can be used for both transient and stable expression of genes
  • Note: For stable expression, the pCI Vector must also be transfected with an expression vector containing a selectable gene for mammalian cells
pCI-neo Mammalian Expression Vector
  • Similar to pCI Mammalian Expression Vector
  • Also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells
  • Can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G-418
Both Vectors
  • Can also be used to synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication