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Promega™ P450-Glo™ CYP1A2 Induction/Inhibition Assay DFS Item

The P450-Glo™ CYP1A2 Assay and Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays

Manufacturer:  Promega™ V8421

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Catalog No. PRV8421

Includes: Luciferin-1A2 (30μL), 500X D-Cysteine (100μL), luciferin detection reagent and reconstitution buffer.



A conventional cytochrome P450 reaction is performed by incubating the cytochrome P450 and a luminogenic cytochrome P450 substrate. The substrates of the P450-Glo Assays are derivatives of beetle luciferin, which are not substrates for luciferase but are converted by cytochrome P450s to luciferin, which in turn reacts with luciferase to produce light. The amount of light produced is directly proportional to cytochrome P450 activity.

  • Contains Luciferin-1A2
  • For use in cell-based applications
  • Measures CYP Inhibition, recombinant CYP activity, native CYP activity, CYP induction
  • Rapid method provides greater sensitivity
  • No fluorescence interference yields low false-positive rate and signal stability
  • Features cell permeability, DMSO tolerance
  • Scalable to 384-well format

  • Measure recombinant CYP1A2 activities in membrane fractions from heterologous expression systems, such as Sf9 cells and E. coli
  • Measure native CYP1A2 activity in cells and microsomal fractions from cells and tissues
  • Screen drugs and new chemical entities for their capacity to modulate CYP450 activities in native or recombinant fractions



Homogeneous, Luminescent
1 x 30μL Luciferin-1A2, 1 x 100μL D-Cysteine 500X, 1 x 1 each Luciferin detection reagent, 1 x 10mL Reconstitution Buffer
−30 to −10°C
For 384-well format
For measuring cytochrome P450 activity