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Promega™ ONE-Glo™ Luciferase Assay System


Provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells

Manufacturer: promega™  E6110

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Catalog No. PRE6110

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Description & Specifications

Specifications

Detection Method Luminescence
Includes 1 x 10mL ONE-Glo™ Luciferase Assay Buffer, 1 x 1 vial ONE-Glo™ Luciferase assay substrate
pH 6.7
For Use With (Application) Detection of firefly luciferase reporter gene expression in mammalian cells
For Use With (Equipment) 384- and 1536-well plates
Quantity 10mL
Storage Requirements -20°C

  • Utilizes a novel luciferase substrate (5'-fluoroluciferin) that is less sensitive to culture media, phenol red, and luciferase inhibitors than other luciferase reagents
  • Generates an extended, bright light output that allows high sensitivity, especially when extended incubation is required prior to reading results
  • Less sensitive to mixing and dispensing conditions, enhancing reproducibility and making the assay ideal for use in 384- and 1536-well microplates
  • Offers extended stability at room temperature and 4°C for more convenient everyday use