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Promega™ Nonradioactive Phosphatase Assay Systems


Fast, flexible alternative for measuring protein phosphatase activity

Manufacturer: promega™  V2471

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Catalog No. PR-V2471

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Description & Specifications

Specifications

Product Type Tyrosine system
Quantity 96 Reactions
For Use With (Application) Can be used with partially fractionated and purified samples as well as in crude cell or tissue extracts
Storage Requirements 4°C

  • Measure the absorbance of molybdate:malachite green:phosphate complex
  • Can be used with partially fractionated and purified samples as well as in crude cell or tissue extracts
  • No need to prelabel substrates; supplied substrate is stable for six months with proper storage and handling
  • Concentration of substrates and working range of assay are compatible with Vmax and Km values of commonly used phosphatases using phosphopeptide substrates
  • Effective range for detection of phosphate is 100–4000pmol
  • Allow the use of a variety of buffer conditions and substrates
  • Serine/Threonine System contains RRA(pT)VA, which is compatible with several Ser/Thr phosphatases (supplied phosphopeptide is a poor substrate for Protein Phosphatase I)
  • Tyrosine System contains two phosphopeptides, END(pY)INSAL (4) and DADE(pY)LIPQQG