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Promega™ Nonradioactive Phosphatase Assay Systems DFS Item

The Non-Radioactive Phosphatase Assay Systems provide a fast, convenient and flexible alternative for measuring protein phosphatase activity.

Manufacturer:  Promega™ V2471

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Catalog No. PR-V2471



Description

Description

  • Measure the absorbance of molybdate:malachite green:phosphate complex
  • Can be used with partially fractionated and purified samples as well as in crude cell or tissue extracts
  • No need to prelabel substrates; supplied substrate is stable for six months with proper storage and handling
  • Concentration of substrates and working range of assay are compatible with Vmax and Km values of commonly used phosphatases using phosphopeptide substrates
  • Effective range for detection of phosphate is 100–4000pmol
  • Allow the use of a variety of buffer conditions and substrates
  • Serine/Threonine System contains RRA(pT)VA, which is compatible with several Ser/Thr phosphatases (supplied phosphopeptide is a poor substrate for Protein Phosphatase I)
  • Tyrosine System contains two phosphopeptides, END(pY)INSAL (4) and DADE(pY)LIPQQG

Specifications

Specifications

Tyrosine system
96 Reactions
Can be used with partially fractionated and purified samples as well as in crude cell or tissue extracts
2 to 10°C
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