Please login to your online account to display your discounted pricing

Promega™ Nonradioactive Phosphatase Assay Systems

Fast, flexible alternative for measuring protein phosphatase activity

$616.32 - $616.32

Specifications

Quantity 96 Reactions
For Use With (Application) Can be used with partially fractionated and purified samples as well as in crude cell or tissue extracts
Storage Requirements 4°C
View More Specs
Products
Catalog Number Mfr. No. Product Type Price Quantity    

PR-V2471

 
promega™
V2471
Tyrosine system Each for $616.32

PR-V2460

 
promega™
V2460
Ser or Thr system Each for $616.32
Description & Specifications

Specifications

Quantity 96 Reactions
For Use With (Application) Can be used with partially fractionated and purified samples as well as in crude cell or tissue extracts
Storage Requirements 4°C

  • Measure the absorbance of molybdate:malachite green:phosphate complex
  • Can be used with partially fractionated and purified samples as well as in crude cell or tissue extracts
  • No need to prelabel substrates; supplied substrate is stable for six months with proper storage and handling
  • Concentration of substrates and working range of assay are compatible with Vmax and Km values of commonly used phosphatases using phosphopeptide substrates
  • Effective range for detection of phosphate is 100–4000pmol
  • Allow the use of a variety of buffer conditions and substrates
  • Serine/Threonine System contains RRA(pT)VA, which is compatible with several Ser/Thr phosphatases (supplied phosphopeptide is a poor substrate for Protein Phosphatase I)
  • Tyrosine System contains two phosphopeptides, END(pY)INSAL (4) and DADE(pY)LIPQQG