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Promega™ NanoLuc™ pNL2 Vectors

Configured with NanoLuc Luciferase for use in reporter gene assays of transcriptional control—ideal for cloning of known or putative promoter region and establishment of stable cell line through Hygromycin selection

$535.00 - $535.00

Specifications

Format Liquid
pH 8
Quantity 20μg
Storage Requirements -20°C
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Catalog Number Mfr. No. Quantity Product Type Price Quantity    

PRN1061

 
promega™
N1061
20μg pNL2.1(Nluc/Hygro) Each for $535.00
Description & Specifications

Specifications

Format Liquid
pH 8
Quantity 20μg
Storage Requirements -20°C

Multiple forms of luciferase have been configured to meet differing experimental objectives. Unfused Nluc offers maximal light output and sensitivity, NanoLuc-PEST (NlucP) closely couples protein expression to changes in transcriptional activity and increased signal-to background ratios, and NanoLuc luciferase fused to an N-terminal secretion signal (secNluc) is suitable when a secreted reporter is preferred.

NanoLuc (Nluc) Luciferase Technology:

  • NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter
  • Luminescent reaction is ATP-independent and designed to suppress background luminescence for maximal assay sensitivity
  • Luminescence is linearly proportional to amount of NanoLuc protein over 1,000,000-fold concentration range
  • Signal half-life ≥2 hours when detected with Nano-Glo™ Luciferase Assay Reagent
Nanoluc Luciferase Enzyme:
  • Monomeric, with 171 amino acids and 513 base pairs
  • With high thermal stability and broad pH range
  • No post-translational modifications or disulfide bonds
  • Uniform distribution in cells
  • Emission spectrum well suited for bioluminescence resonance energy transfer
Nluc Vector:
  • Uses pGL4-based backbone for easy sequence transfer from existing plasmids
  • Reduces anomalous results by removing many transcription factor binding sites and other potential regulatory elements
  • Nluc gene variations are codon optimized and have many potential regulatory elements or other undesirable features removed, such as common restriction enzyme sites

Transcription regulation, Virus-cell interactions, Compound screening, Post-translational modifications, GPCR signaling, Cell signaling, Promoter analysis