Promega NanoLuc™ pNL2 Vectors 

Description

Multiple forms of luciferase have been configured to meet differing experimental objectives. Unfused Nluc offers maximal light output and sensitivity, NanoLuc-PEST (NlucP) closely couples protein expression to changes in transcriptional activity and increased signal-to background ratios, and NanoLuc luciferase fused to an N-terminal secretion signal (secNluc) is suitable when a secreted reporter is preferred.

NanoLuc (Nluc) Luciferase Technology:

• NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter
• Luminescent reaction is ATP-independent and designed to suppress background luminescence for maximal assay sensitivity
• Luminescence is linearly proportional to amount of NanoLuc protein over 1,000,000-fold concentration range
• Signal half-life ≥2 hours when detected with Nano-Glo™ Luciferase Assay Reagent

• Monomeric, with 171 amino acids and 513 base pairs
• With high thermal stability and broad pH range
• No post-translational modifications or disulfide bonds
• Uniform distribution in cells
• Emission spectrum well suited for bioluminescence resonance energy transfer

• Uses pGL4-based backbone for easy sequence transfer from existing plasmids
• Reduces anomalous results by removing many transcription factor binding sites and other potential regulatory elements
• Nluc gene variations are codon optimized and have many potential regulatory elements or other undesirable features removed, such as common restriction enzyme sites

Transcription regulation, Virus-cell interactions, Compound screening, Post-translational modifications, GPCR signaling, Cell signaling, Promoter analysis