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Promega™ NADP/NADPH-Glo™ Assay


Bioluminescent, homogeneous, single-reagent-addition method for rapid detection of total oxidized and reduced NADP+ and NADPH and determining their ratio in biological samples and defined enzyme reactions

Manufacturer: promega™  G9081

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Catalog No. PRG9081

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Description & Specifications

Specifications

Product Type NADP/NADPH-Glo assay kit
Quantity 10mL

  • High Sensitivity: enables detection of total NADP+ and NADPH directly in the wells; fewer cells are required, with no sample preparation
  • Homogeneous, One-Step Protocol: Total NADP+ and NADPH is measured directly in wells of 96- or 384-well cell culture plate with one reagent addition
  • Large Assay Window: Detects 10nM to 400nM NADP+ or NADPH; 100nM with a signal higher than fivefold over background and an assay window (maximum signal-to-background ratio) of ≥100
  • Automation Compatible: Compatible with automated and high-throughput protocols. Reactions are scalable and can be performed at low volumes in 96-, 384- and 1536-well plates
  • Reliability and Reproducibility: Routinely yields Z« factors >0.7
  • Luminescence-Based NADP+ and NADPH Detection: Luminescent format avoids fluorescent interference due to reagents and test compounds sometimes seen in fluorescent assays

G9081 assay kit contains sufficient reagents: For 100 reactions in 96-well plates, 400 assays in 384-well plates or 2,000 assays in low-volume 384-well plates. Assay volumes can be varied depending on plate format as long as a 1:1 ratio of sample to NADP/NADPH-Glo™ Detection Reagent is maintained
G9082 assay kit contains sufficient reagents: For 500 reactions in 96-well plates, 2,000 assays in 384-well plates or 10,000 assays in low-volume 384-well plates. Assay volumes can be varied depending on plate format as long as a 1:1 ratio of sample to NADP/NADPH-Glo™ Detection Reagent is maintained