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Promega™ MultiTox-Glo™ Multiplex Cytotoxicity Assay


Fluorescent and luminescent assay sequentially measures two protease activities—one a marker of viability, the other a marker of cytotoxicity

Manufacturer: promega™  G9270

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Catalog No. PRG9270

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Description & Specifications

Specifications

Quantity 10mL
Storage Requirements -20°C
Detection Method Fluorescence, Luminescence
Sufficient For 100 x 96-well assays and 400 x 384-well assays

The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant peptide substrate (GF-AFC), which enters intact cells and is cleaved by the live-cell protease to generate a fluorescent signal proportional to the number of viable cells. A second, luminogenic cell-impermeant peptide substrate (AAF-aminoluciferin) is used to measure the activity of the dead-cell protease, which is released from cells that have lost membrane integrity and cleaves the substrate to generate a luminescent signal.

  • The number of live cells and dead cells in culture is measured using a homogeneous “add-mix-measure” protocol
  • The ratio of the number of live cells to the number of dead cells is independent of cell number and can be used to normalize data, making results more comparable from well-to-well, plate-to-plate, and day-to-day
  • Independent cell viability and cytotoxicity measurements serve as controls for each other, permitting the identification of more false-positives and false-negatives