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Promega™ MultiTox-Glo™ Multiplex Cytotoxicity Assay DFS Item

The MultiTox-Glo™ Multiplex Cytotoxicity Assay is a fluorescent and luminescent assay that measures the relative number of live and dead cells in a population.

Manufacturer:  Promega™ G9270

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Catalog No. PRG9270

Includes: Assay buffer, AAF-Glo™ substrate, GF-AFC substrate



The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant peptide substrate (GF-AFC), which enters intact cells and is cleaved by the live-cell protease to generate a fluorescent signal proportional to the number of viable cells. A second, luminogenic cell-impermeant peptide substrate (AAF-aminoluciferin) is used to measure the activity of the dead-cell protease, which is released from cells that have lost membrane integrity and cleaves the substrate to generate a luminescent signal.

  • The number of live cells and dead cells in culture is measured using a homogeneous “add-mix-measure” protocol
  • The ratio of the number of live cells to the number of dead cells is independent of cell number and can be used to normalize data, making results more comparable from well-to-well, plate-to-plate, and day-to-day
  • Independent cell viability and cytotoxicity measurements serve as controls for each other, permitting the identification of more false-positives and false-negatives



−30 to −10°C
Fluorescence, Luminescence
100 x 96-well assays and 400 x 384-well assays


Patent Pending.
U.S. Patents 6,602,677 and 7,241,584, Australian Patents 754312 and 785294 and other patents and patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673.