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Promega™ Mass Spec Grade Trypsin/Lys-C Mix


Designed to improve digestion of proteins or protein mixtures in solution. Mass Spec Grade Trypsin/Lys-C Mix is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade.

Manufacturer: promega™  V5073

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Catalog No. PRV5073

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Description & Specifications

Specifications

Product Name Trypsin/Lys-C Mix
Format Liquid
Color Colorless
Includes 5 x 20μg Mass spec grade lyophilized Trypsin/Lys-C Mix, 500μL Resuspension Buffer
Quantity 5 x20μg
For Use With (Application) In-solution Protein digestion, in-solution digestion of proteolytically resistant Proteins
Storage Buffer 50mM Acetic Acid (pH <3) Resuspension Buffer
Storage Requirements -30° to -10°C

  • Simple to Use: Use standard overnight digestion with nondenaturing conditions
  • Enhanced Proteolysis: Increase peptide recovery, resulting in enhanced protein quantitation and improved reproducibility and eliminating the majority of missed cleavages
  • Tolerant to Trypsin-Inhibiting Contaminants: Generate mass spectrometry data from poor-quality sample material
  • Enhances digestion and compensates for trypsin proteolytic inefficiency at lysine sites
  • Provides greater tolerance to trypsin-inhibiting agents, assuring efficient digestion of proteins for which protein purification is limited or not feasible
  • Applications:
  • In-solution protein digestion
  • In-solution digestion of proteolytically resistant proteins