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Promega™ MagneHis™ Protein Purification System DFS Item

The MagneHis Protein Purification System provides a magnetic-based, rapid method for the purification of polyhistidine- or HQ-tagged, expressed proteins.

Manufacturer:  Promega™ V8500

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Catalog No. PR-V8500

Includes: MagneHis binding/wash buffer, 50mL; FastBreak™ cell lysis reagent, 15mL; DNase I, lyophilized, 1 vial; MagneHis elution buffer, 10mL; MagneHis Ni-particles, 2mL



  • Utilizes paramagnetic precharged MagneHis Ni-Particles to isolate polyhistidine-tagged protein directly from crude cell lysate Binds up to 1mg of polyhistidine-tagged protein per 1mL of MagneHis Ni-Particles Compatible with a manual or automated protocol
  • Contains all necessary reagents for purification

MagneHis Ni-Particles are also available separately.

U.S. Pat. Nos. 7,112,552; 7,304,015; 7,304,016; 7,354,750; 6,174,704; and 7,319,021 and other patents and patents pending.
Certain applications of this product are covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.



Rapid and reliable purification of Polyhistidine-tagged Proteins from 1mL to 1L of bacterial culture
Protein Purification System
2 to 10°C
50mL MagneHis binding/Wash Buffer, 15mL FastBreak™ cell lysis reagent, DNase I, 1 vial lyophilized, 10mL MagneHis Elution Buffer, 2mL MagneHis Ni-particles
65 Reactions