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Promega™ MagneGST™ Protein Purification System

Simple, rapid, and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins from a crude or cleared lysate

$250.38 - $250.38

Specifications

For Use With (Application) Protein purification of Glutathione-S-transferase fusion Proteins from cleared or crude E. coli cell lysate, high-throughput purification of GST-tagged Proteins using auto mated platforms
Includes 4mL MagneGST™ Glutathione particles, 2mL Glutathione, 80mL MagneGST™ binding/Wash Buffer, 12mL MagneGST™ cell lysis reagent, 1000μL RQ1 RNase-free DNase, 2mL Tris Buffer, 0.5M (pH 8.1)
Storage Requirements 4° or -70°C
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Includes:  MagneGST Glutathione Particles (also available separately), (4mL); Glutathione, (2mL); MagneGST Binding/Wash Buffer, (80mL); MagneGST Cell Lysis Reagent, (12mL); RQ1 RNase-free DNase, (1000μL); Tris Buffer, 0.5M (pH 8.1), (2mL)
Requires:  Magnetic separation stand, such as the MagneSphere Technology Magnetic Separation Stand (Mfr. No. Z5342).
Products
Catalog Number Mfr. No. Product Type Quantity Price Quantity    

PR-V8600

 
promega™
V8600
Protein Purification System 40 Reactions Each for $250.38
Description & Specifications

Specifications

For Use With (Application) Protein purification of Glutathione-S-transferase fusion Proteins from cleared or crude E. coli cell lysate, high-throughput purification of GST-tagged Proteins using auto mated platforms
Includes 4mL MagneGST™ Glutathione particles, 2mL Glutathione, 80mL MagneGST™ binding/Wash Buffer, 12mL MagneGST™ cell lysis reagent, 1000μL RQ1 RNase-free DNase, 2mL Tris Buffer, 0.5M (pH 8.1)
Storage Requirements 4° or -70°C

  • Provides one-step purification of multiple samples
  • Scalable from 1mL of culture to more than 50mL of culture
  • Produces high yields with little or no nonspecific background
  • Does not require high-speed centrifugation after cell lysis to clear lysate
  • Compatible with manual or automated protocols

This product or portions thereof is manufactured under license from Amrad Corporation Limited. For non-commercial research use only. All other uses require a license from Amrad Corporation Limited, Unit 6, 663 Victoria Street, Abbotsford, Victoria 3067, Australia, under U.S. Patent 5,654,176, Australian Patent 607511, Canadian Patent 1338903 and other issued patents.
Patent pending.
This product is licensed for use under U.S. Patent 6,174,704.
Certain applications of this product are covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.