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Promega™ MagneGST™ Protein Purification System DFS Item

Simple, rapid, and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins from a crude or cleared lysate

Manufacturer:  Promega™ V8600

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Catalog No. PR-V8600


 

Requires : Magnetic separation stand, such as the MagneSphere Technology Magnetic Separation Stand (Mfr. No. Z5342).

Specifications

Description

MagneGST Protein Purification Kit; Utilizes immobilized glutathione paramagnetic particles to isolate GST-tagged protein directly from crude or cleared lysate using a manual or automated procedure; 40 rxns

  • Provides one-step purification of multiple samples
  • Scalable from 1mL of culture to more than 50mL of culture
  • Produces high yields with little or no nonspecific background
  • Does not require high-speed centrifugation after cell lysis to clear lysate
  • Compatible with manual or automated protocols

Description & Specifications

Specifications

4mL MagneGST™ Glutathione particles, 2mL Glutathione, 80mL MagneGST™ binding/Wash Buffer, 12mL MagneGST™ cell lysis reagent, 1000μL RQ1 RNase-free DNase, 2mL Tris Buffer, 0.5M (pH 8.1)
Protein purification of Glutathione-S-transferase fusion Proteins from cleared or crude E. coli cell lysate, high-throughput purification of GST-tagged Proteins using auto mated platforms
40 Reactions
4° or -70°C
Protein Purification System