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Promega™ M-MLV Reverse Transcriptase

RNA-dependent DNA polymerase can be used in primer extension or cDNA synthesis with >5kb mRNA templates

Manufacturer: promega™  M5313

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Catalog No. PR-M5313

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Description & Specifications


For Use With (Application) First-strand cDNA synthesis, primer extension
Includes 2 x 1mL Buffer pack
Product Name M-MLV Reverse Transcriptase Buffer Pack
Quantity 2 x 1mL
Format Liquid
Concentration 5X
Source Recombinant E. coli Strain
Storage Buffer Tris-HCl (pH 7.5 at 25°C) 20mM, NaCl 0.2M, EDTA 0.1mM, DTT 1mM, 0.01% Nonidet™ P40™ and Glycerol 50%
pH 8.3
Storage Requirements -20°C

  • Exhibits weaker RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase
  • May be inactivated by heating at 70°C for 10 minutes
  • Produced by the pol gene of Moloney Murine Leukemia Virus
  • Provided with 5X Reaction Buffer (250mM Tris-HCl [pH 8.3 at 25°C], 375mM KCl, 15mM MgCl2, 50mM DTT); buffer also available separately