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Promega™ LigaFast™ Rapid DNA Ligation System DFS Item

Designed to eliminate the need for further purification prior to transformation of ligated DNA

$94.00 - $318.00

Specifications

Formulation Tris HCl (pH 7) 10mM, KCl 50mM, DTT 1mM, EDTA 0.1mM, Glycerol 50%
For Use With (Application) 5′, 3′ or blunt ended DNA inserts
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Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity pH Price Quantity  
Catalog Number Mfr. No. Quantity pH Price Quantity  
PR-C6711 Promega™
C6711
3 x 500μL 7.8 Each for $94.00
PR-M8221 Promega™
M8221
40 Reactions 7.8 Each for $97.00
PR-M8225 Promega™
M8225
150 Reactions 7.4 Each for $318.00
Specifications

Description

  • Use with 5', 3' or blunt-ended DNA inserts
  • Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes at room temperature
  • No requirement to purify ligated DNA prior to heat-shock transformation in E. coli ligations conducted at room temperature
  • No additional buffer modifications required prior to use
  • Ligations performed using the LigaFast™ system are comparable to standard overnight ligations
  • Blue/White Cloning Qualified

Description & Specifications

Specifications

Tris HCl (pH 7) 10mM, KCl 50mM, DTT 1mM, EDTA 0.1mM, Glycerol 50%
5′, 3′ or blunt ended DNA inserts