missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Promega™ LigaFast™ Rapid DNA Ligation System DFS Item

Designed to eliminate the need for further purification prior to transformation of ligated DNA

Manufacturer:  Promega™ M8225

 View more versions of this product

Catalog No. PR-M8225



Description

Description

  • Use with 5', 3' or blunt-ended DNA inserts
  • Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes at room temperature
  • No requirement to purify ligated DNA prior to heat-shock transformation in E. coli ligations conducted at room temperature
  • No additional buffer modifications required prior to use
  • Ligations performed using the LigaFast™ system are comparable to standard overnight ligations
  • Blue/White Cloning Qualified

Specifications

Specifications

5′, 3′ or blunt ended DNA inserts
Tris HCl (pH 7) 10mM, KCl 50mM, DTT 1mM, EDTA 0.1mM, Glycerol 50%
150 Reactions
Fluid
7.4
−30 to −10°C
Documents
Promotions

Promotions