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Promega™ LigaFast™ Rapid DNA Ligation System

Designed to eliminate the need for further purification prior to transformation of ligated DNA

Manufacturer: promega™  M8221

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Catalog No. PR-M8221

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Description & Specifications


For Use With (Application) 5′, 3′ or blunt ended DNA inserts
Format Fluid
Formulation Tris HCl (pH 7) 10mM, KCl 50mM, DTT 1mM, EDTA 0.1mM, Glycerol 50%
pH 7.8
Quantity 40 Reactions
Storage Requirements -20°C

  • Use with 5', 3' or blunt-ended DNA inserts
  • Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes at room temperature
  • No requirement to purify ligated DNA prior to heat-shock transformation in E. coli ligations conducted at room temperature
  • No additional buffer modifications required prior to use
  • Ligations performed using the LigaFast™ system are comparable to standard overnight ligations
  • Blue/White Cloning Qualified