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Promega LigaFast™ Rapid DNA Ligation System 
Designed to eliminate the need for further purification prior to transformation of ligated DNA
Supplier: Promega M8221
Description
- Use with 5', 3' or blunt-ended DNA inserts
- Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes at room temperature
- No requirement to purify ligated DNA prior to heat-shock transformation in E. coli ligations conducted at room temperature
- No additional buffer modifications required prior to use
- Ligations performed using the LigaFast™ system are comparable to standard overnight ligations
- Blue/White Cloning Qualified
Specifications
| -30°C to -10°C | |
| 5′, 3′ or blunt ended DNA inserts | |
| 7.8 |
| Tris HCl (pH 7) 10mM, KCl 50mM, DTT 1mM, EDTA 0.1mM, Glycerol 50% | |
| 30 | |
| Fluid |