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Promega™ Klenow Fragment, DNA Polymerase I DFS Item

A DNA-dependent DNA polymerase that lacks the 5′→3′ exonuclease activity of intact E. coli DNA Polymerase I but retains its 5′→3′ polymerase, 3′→5′ exonuclease and strand displacement activities.

Manufacturer:  Promega™ M2201

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Catalog No. PR-M2201



Specifications

Specifications

DNA Polymerase I Large (Klenow) Fragment
5U/μL
10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT
>80%
68
Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method
−30 to −10°C
Liquid
Colorless
Recombinant E. coli strain
7.2
150U
10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
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