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Promega™ Kinase-Glo™ Plus Luminescent Kinase Assay System


Homogeneous, high-throughput screening (HTS) method of measuring kinase activity

Manufacturer: promega™  V3774

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Catalog No. PR-V3774

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Description & Specifications

Specifications

Product Type Kinase-Glo Plus
Format Fluid
pH 7
For Use With (Application) Automated high throughput screening and they can be used to assay protein, lipid and sugar kinases
Quantity 10 × 100mL
Storage Requirements -20°C

Quantifies amount of ATP remaining in solution following a kinase reaction. Easily performed in a single well of a 96- or 384-well plate: simply add volume of Kinase-Glo Plus reagent equal to volume of completed kinase reaction, then measure luminescence.

  • Ideal for high-throughput analysis of library compounds to identify kinase inhibitors
  • Compatible with virtually any kinase/kinase substrate combination
  • Can be performed at higher ATP concentrations than traditional Kinase-Glo assay
  • Suitable for screening non-ATP binding site inhibitors: Kinase-Glo Plus Assay is linear to 100μM ATP, making it well-suited for use with kinases with high Km for ATP as well as for screening for kinase inhibitors that do not compete at the ATP binding site
  • Can be used for a wide variety of kinases (lipid, sugar, and alcohol) and substrates (peptides, proteins, lipids, sugars, alcohols)
  • Less susceptible to interference than traditional luciferase-based ATP detection reagents
  • Nonradioactive