Promega™ Kinase-Glo™ Max Luminescent Kinase Assay
The Kinase-Glo™ Luminescent Kinase Assays are homogeneous non-radioactive systems for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction.
Manufacturer: Promega™ V6073
- Quantifies the amount of ATP remaining in solution following a kinase reaction1, 2
- Designed for use with multiwell plate formats, making it ideal for high throughput screening
- Can be used with a wide range of kinases, including lipid kinases and substrates
- Features low susceptibility to library compounds; Z' values are >0.8 in either 96- or 384-well formats
1U.S. Patent 6,602,677, Australian Patent 754312 and other patents pending.
2The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024, 5,674,713 and 5,700,673.
|Homogeneous, Nonradioactive Method|
|1 x 100mL Kinase-Glo™ max Buffer, 1 x resuspend to 100mL Kinase-Glo™ max substrate|
|Kinase-Glo Max Luminescent Kinase Assay|
|−30 to −10°C|
|For multiwell plates|
|For determining the activity of purified Kinases by quantifying the amount of ATP|