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Promega™ ImProm-II™ Reverse Transcription System


Formulated for stand-alone applications or for easy transition to gene-specific target amplification

Manufacturer: promega™  A3800

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Catalog No. PR-A3800

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Description & Specifications

Specifications

For Use With (Application) Reverse transcription of RNA templates from total RNA, poly(A)+ mRNA, or synthetic transcript RNA
Format Liquid
Includes Upstream control primer 100μL, Downstream control primer 100μL, MgCl2 1.2mL, Oligo(dT)15 Primer 50μg, dNTP mix 320μL, Random primers 50μg, 1.2kb kanamycin positive control RNA 5μg, ImProm-II 5X reaction buffer 600μL, ImProm-II reverse transcriptase 100μL, Recombinant RNasin ribonuclease inhibitor 2500U, Nuclease-free water 2 × 1250μL
pH 7
Product Type ImProm-II Reverse Transcription System
Quantity 100 Reactions

  • Reverse transcribes RNA templates from total RNA, poly(A)+ mRNA, or synthetic transcript RNA
  • Enables robust, full-length cDNA synthesis for the analysis of rare or long messages (up to 8.9kb)
  • Uses volumes of 1–20μL from the initial RT reaction in coupled or uncoupled PCR reactions
  • May also be used for the incorporation of regular, Cy™3-modified, Cy5-modified, and amino-allyl-modified nucleotides for microarray applications
  • Refill components available separately