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Promega™ Immobilized Trypsin


For effective removal of trypsin, chymotrypsin and elastase from protein digests

Manufacturer: promega™  V9013

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Catalog No. PRV9013

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Description & Specifications

Specifications

For Use With (Application) For effective removal of Trypsin, Chymotrypsin and Elastase from Protein digests
Includes 4mL Immobilized Trypsin, Spin column with caps (20 Each)
Product Type Immobilized Trypsin cell lysis reagent
Quantity 2mL
Sufficient For 10 Reactions
Storage Requirements 4°C

Digested peptides are easily separated from the Immobilized Trypsin as they flow through the spin column into the collection tube. Immobilized Trypsin is easily removed from the peptide solution because the trypsin does not pass though the column frit. Trypsin is a proteolytic enzyme, which cleaves at the carboxyl side of positively charged Lysine (Lys) and Arginine (Arg). When these amino acids are followed by the nonpolar Proline (Pro), the digestion of the site is not efficient. When Lys and Arg are followed by acids [Aspartic Acid (Asp) and Glutamic Acid (Glu)] the digestion is also not as efficient.

  • Fast: Digestions can be accomplished in as little as 30 minutes
  • Scalable: Easily adjustable protocol to accommodate various protein concentrations
  • Easy-to-Use: No shaking or water baths necessary