Please login to your online account to display your discounted pricing

Promega™ Immobilized Trypsin

For effective removal of trypsin, chymotrypsin and elastase from protein digests

$262.15 - $479.36

Specifications

Product Type Immobilized Trypsin cell lysis reagent
Quantity 2mL
For Use With (Application) For effective removal of Trypsin, Chymotrypsin and Elastase from Protein digests
Storage Requirements 4°C
Sufficient For 10 Reactions
View More Specs
Products
Catalog Number Mfr. No. Includes Price Quantity    

PRV9012

 
promega™
V9012
2mL Immobilized Trypsin, Spin column with caps (10 Each) Each for $262.15

PRV9013

 
promega™
V9013
4mL Immobilized Trypsin, Spin column with caps (20 Each) Each for $479.36
Description & Specifications

Specifications

Product Type Immobilized Trypsin cell lysis reagent
Quantity 2mL
For Use With (Application) For effective removal of Trypsin, Chymotrypsin and Elastase from Protein digests
Storage Requirements 4°C
Sufficient For 10 Reactions

Digested peptides are easily separated from the Immobilized Trypsin as they flow through the spin column into the collection tube. Immobilized Trypsin is easily removed from the peptide solution because the trypsin does not pass though the column frit. Trypsin is a proteolytic enzyme, which cleaves at the carboxyl side of positively charged Lysine (Lys) and Arginine (Arg). When these amino acids are followed by the nonpolar Proline (Pro), the digestion of the site is not efficient. When Lys and Arg are followed by acids [Aspartic Acid (Asp) and Glutamic Acid (Glu)] the digestion is also not as efficient.

  • Fast: Digestions can be accomplished in as little as 30 minutes
  • Scalable: Easily adjustable protocol to accommodate various protein concentrations
  • Easy-to-Use: No shaking or water baths necessary