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Promega™ HisLink™ Protein Purification Resins DFS Item

HisLink Protein Purification Resin is a macroporous silica resin modified to contain a high level of tetradentate-chelated nickel (>20mmol Ni/ml settled resin) for efficient capture and purification of bacterially expressed His- or HQ-tagged proteins.

Manufacturer:  Promega™ V8821

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Catalog No. PR-V8821



  • High binding capacity: >15mg/mL
  • Macroporous silica resin modified to contain a high level of tetradentate chelated nickel (>20mmol Ni/mL settled resin)
  • Easily separated from the lysate without filtration or centrifugation
  • May be used in standard gravity column chromatography or automated applications
  • Also suitable for general applications requiring an immobilized metal affinity chromatography matrix (IMAC)

U.S. Patent No. 7,112,552 and other patents pending.
Certain applications of this product are covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.



Purification of His-tagged Proteins from cleared or crude bacterial lysates
HisLink™ Protein Purification Resin
2 to 10°C