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Promega™ HisLink™ 96 Protein Purification System


Provides a simple, quick and robust method of purifying multiple polyhistidine- or HQ-tagged expressed proteins from E. coli using a vacuum-based method

Manufacturer: promega™  V3681

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96-place vacuum manifold

Catalog No. PR-V3681

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Description & Specifications

Specifications

For Use With (Application) Quick and robust method of purifying multiple Polyhistidine-or HQ-tagged expressed Proteins from E. coli using a vacuum-based method
Product Type HisLink™ 96 Protein Purification System
Quantity 5 x 96 Tests
Storage Requirements 4°C

Using the provided FastBreak™ Cell Lysis Reagent, bacterial cells containing polyhistidine- or HQ-tagged protein are lysed directly in culture. The HisLink Resin is added directly to the lysate, and the polyhistidine- or HQ-tagged proteins bind in a matter of minutes. Samples are then transferred to a filtration plate, unbound proteins are washed away and the target protein is recovered by elution with imidazole.

  • Designed to purify expressed polyhistidine- or HQ-tagged proteins directly from deep-well, 96-well plates
  • System is amenable to manual or automated methods, such as the Beckman Coulter Biomek™ 2000 or FX for high-throughput applications
  • No centrifugation required; lysis buffer is added directly to cells in culture medium
  • No long lysozyme incubations are required for cell lysis
  • Perform purification manually or on an automated platform
  • Binding capacity of 1mg of polyhistidine-tagged protein per well