Patent Pending. This product is licensed for use under U.S. Pat. No. 6,174,704. Certain applications of this product are covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.
Using the provided FastBreak™ Cell Lysis Reagent, bacterial cells containing polyhistidine- or HQ-tagged protein are lysed directly in culture. The HisLink Resin is added directly to the lysate, and the polyhistidine- or HQ-tagged proteins bind in a matter of minutes. Samples are then transferred to a filtration plate, unbound proteins are washed away and the target protein is recovered by elution with imidazole.
Designed to purify expressed polyhistidine- or HQ-tagged proteins directly from deep-well, 96-well plates
System is amenable to manual or automated methods, such as the Beckman Coulter Biomek™ 2000 or FX for high-throughput applications
No centrifugation required; lysis buffer is added directly to cells in culture medium
No long lysozyme incubations are required for cell lysis
Perform purification manually or on an automated platform
Binding capacity of 1mg of polyhistidine-tagged protein per well