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Promega™ HaloTag™ Vectors


A variety vectors for mammalian, E. coli, and cell-free protein expression

Manufacturer: promega™  G2871

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Catalog No. PRG2871

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Description & Specifications

Specifications

Concentration 100ng/μL
For Use With (Application) Cell imaging of Protein localization or trafficking in conjunction with the fluorescent HaloTag™ ligands by using expression of HaloTag™ fusion Proteins in E. coli and cell-free Protein expression
Promoter CMVd2
Protein Expression Level Low
Quantity 20μg
Vector pFN23K HaloTag™ CMVd2 Flexi™ vector
Storage Requirements -20°C
Storage Buffer 10mM Tris HCl (pH 8), 1mM EDTA

  • All vectors are compatible with Promega Flexi Vector System, directional cloning method for protein-coding sequences
  • Vectors designated “pFC” are configured to append HaloTag 7 to carboxy-terminus of protein fusion partner
  • Vectors designated “pFN” are configured to append HaloTag 7 to amino-terminus of protein fusion partner

Vectors for Mammalian Protein Expression

  • Vectors contain either full-length (pFC14, pFN21) or modified (pFC15, pFC16, pFC17, pFN22, pFN23, pFN24) human cytomegalovirus (CMV) promoter
  • The modified CMV promoter, which contains various deletions, provides varying levels of protein expression levels, depending on protein of interest and cell type
Vectors for E. coli and Cell-Free Protein Expression
  • pFN18 vectors provide T7 RNA polymerase-driven protein expression in E. coli or in cell-free translation systems
  • pFN19 vectors provide T7 RNA polymerase-driven protein expression in E. coli ( ∽two- to four-fold lower protein expression compared to pFN18 vectors) or T7 or SP6 RNA polymerase-driven protein expression in cell-free translation systems
  • pFC20 vectors provide T7 RNA polymerase-driven protein expression in E. coli or T7 or SP6 RNA polymerase-driven protein expression in cell-free translation systems

Cellular imaging, Protein:protein interaction, Protein:DNA interaction.