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Promega™ HaloTag™ Protein Purification System

Purifies proteins fused to HaloTag, a novel protein tag that enhances the expression and solubility of recombinant proteins

Manufacturer: promega™  G6280

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Catalog No. PRG6280

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Description & Specifications


Quantity Each
Product Type HaloTag™ Protein purification system
Includes HaloLink™ Resin, 100mL of 25% slurry, HisLink Protein purification Resin, 5mL, TEV Protease for use with HaloLink™ Resin, 1.6mL
Storage Requirements 4°C

HaloTag Technology enables the covalent, efficient and specific capture of a protein of interest onto HaloLink™ Resin, overcoming the equilibrium-based limitations associated with affinity tags, including poor capture of proteins expressed at low levels and protein loss during washing of the purification resin.

  • Provides superior yield, purity, and specific activity of soluble, functional proteins compared to His-Tag, GST, and MBP affinity tags due to specific and covalent HaloTag fusion protein capture and immobilization on HaloLink resin
  • Permits recovery of poorly expressed fusion proteins
  • Utilizes one buffer compatible with downstream applications for all purification steps, simplifying the process and saving time
  • Suitable for use with HaloTag fusion proteins expressed in E. coli, as well as HaloTag fusion proteins expressed in mammalian and cell-free systems