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Promega™ HaloTag™ Mammalian Protein Purification System

An optimized kit for purification of HaloTag fusion proteins from mammalian cell culture lysates

$396.97 - $894.52

Specifications

Quantity Each
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Includes:  HaloLink Resin, HaloTag TMRDirect Ligand, Protease Inhibitor Cocktail (50X), HaloTEV Protease, Spin Columns
Products
Catalog Number Mfr. No. For Use With (Application) Includes Storage Requirements Product Type Price Quantity    

PRG6799

 
promega™
G6799
Detection and purification of HaloTag™ fusion Proteins from mammalian cell culture lysates HaloTEV Protease, Protease inhibito r cocktail, HaloLink™ Resin, HaloTag™ TMRDirect™ ligand Each for $396.97

PRG6795

 
promega™
G6795
Detection and purification of HaloTag™ fusion Proteins from mammalian cell culture lysates HaloTEV Protease, Protease inhibito r cocktail, LDH positive control, HaloTag™ TMRDirect™ ligand Each for $894.52
Description & Specifications

Specifications

Quantity Each

HaloTag fusion proteins form a highly specific and covalent bond with the HaloLink™ Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag Mammalian Protein Detection and Purification System includes HaloTag TMRDirect™ Ligand. The simple-to-use fluorescent detection of the HaloTag fusion facilitates rapid optimization of expression and purification conditions.

  • Purify More Protein: HaloLink™ Resin covalently binds >7mg/mL of HaloTag fusion protein (10X more capacity compared to FLAG™). Recovery is highly efficient, commonly >75%
  • Higher Purity: Covalent capture allows extensive and/or stringent washes without loss of bound protein, resulting in very low (<0.1%) nonspecific binding and a highly pure protein
  • Easily Scalable: Scale up and down, important for obtaining mg-plus quantities
  • Optimized for Mammalian Protein Expression: The HaloTag platform allows flexibility to move between purification, pull-downs and cellular imaging with a single construct