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Promega™ HaloTag™ Mammalian Protein Purification System DFS Item

An optimized kit for purification of HaloTag fusion proteins from mammalian cell culture lysates

$416.23 - $938.39

Specifications

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Includes: HaloLink Resin, HaloTag TMRDirect Ligand, Protease Inhibitor Cocktail (50X), HaloTEV Protease, Spin Columns


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Catalog Number Mfr. No. For Use With (Application) Includes Storage Requirements Product Type Price Quantity  
Catalog Number Mfr. No. For Use With (Application) Includes Storage Requirements Product Type Price Quantity  
PRG6799 Promega™
G6799
Detection and purification of HaloTag™ fusion Proteins from mammalian cell culture lysates HaloTEV Protease, Protease inhibito r cocktail, HaloLink™ Resin, HaloTag™ TMRDirect™ ligand Mammalian Protein detection and purification system Each for $416.23
PRG6795 Promega™
G6795
Detection and purification of HaloTag™ fusion Proteins from mammalian cell culture lysates HaloTEV Protease, Protease inhibito r cocktail, LDH positive control, HaloTag™ TMRDirect™ ligand Mammalian Protein detection and purification system Each for $938.39
Specifications

Description

HaloTag fusion proteins form a highly specific and covalent bond with the HaloLink™ Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag Mammalian Protein Detection and Purification System includes HaloTag TMRDirect™ Ligand. The simple-to-use fluorescent detection of the HaloTag fusion facilitates rapid optimization of expression and purification conditions.

  • Purify More Protein: HaloLink™ Resin covalently binds >7mg/mL of HaloTag fusion protein (10X more capacity compared to FLAG™). Recovery is highly efficient, commonly >75%
  • Higher Purity: Covalent capture allows extensive and/or stringent washes without loss of bound protein, resulting in very low (<0.1%) nonspecific binding and a highly pure protein
  • Easily Scalable: Scale up and down, important for obtaining mg-plus quantities
  • Optimized for Mammalian Protein Expression: The HaloTag platform allows flexibility to move between purification, pull-downs and cellular imaging with a single construct

Description & Specifications

Specifications

Each