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Promega™ HaloTag™ Mammalian Protein Purification System


An optimized kit for purification of HaloTag fusion proteins from mammalian cell culture lysates

Manufacturer: promega™  G6795

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Catalog No. PRG6795

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Description & Specifications

Specifications

Product Type Mammalian Protein detection and purification system
Quantity Each
For Use With (Application) Detection and purification of HaloTag™ fusion Proteins from mammalian cell culture lysates
Includes HaloTEV Protease, Protease inhibito r cocktail, LDH positive control, HaloTag™ TMRDirect™ ligand

HaloTag fusion proteins form a highly specific and covalent bond with the HaloLink™ Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag Mammalian Protein Detection and Purification System includes HaloTag TMRDirect™ Ligand. The simple-to-use fluorescent detection of the HaloTag fusion facilitates rapid optimization of expression and purification conditions.

  • Purify More Protein: HaloLink™ Resin covalently binds >7mg/mL of HaloTag fusion protein (10X more capacity compared to FLAG™). Recovery is highly efficient, commonly >75%
  • Higher Purity: Covalent capture allows extensive and/or stringent washes without loss of bound protein, resulting in very low (<0.1%) nonspecific binding and a highly pure protein
  • Easily Scalable: Scale up and down, important for obtaining mg-plus quantities
  • Optimized for Mammalian Protein Expression: The HaloTag platform allows flexibility to move between purification, pull-downs and cellular imaging with a single construct