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Promega™ HaloTag™ Fusion (N-Terminal) Mammalian Expression Vectors DFS Item

Vectors designed for expression of N-terminal-tagged HaloTag™ fusion proteins in mammalian cells. Once expressed, use the HaloTag™ fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag™ Ligands.

Manufacturer:  Promega™ G7721*

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Catalog No. PRG7721



Description

Description

  • Append fluorescent HaloTag tag to the amino-terminus of fused protein
  • Used for transient and stable gene expression, cell imaging of protein localization or trafficking in conjunction
  • Stable expression is mediated by co-expression of neomycin phosphotransferase gene
  • Clone ORF and conveniently transfer these inserts to variety of vectors
  • Available in two types: pHT Vector Series and pF Vector Series

pHT Vector Series

  • Multiple cloning site (MCS) plasmids for traditional cloning
pF Vector Serie
  • Directional cloning method for protein-coding sequences
  • Based on two rare-cutting restriction enzymes, SgfI and PmeI
  • Provide high-fidelity way to transfer protein-coding regions between variety of Flexi Vectors without need to resequence

Flexi Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Flexi Vector Cloning System replaces the barnase gene with insert. These vectors cannot be cultured in normal laboratory strains of E. coli without an insert.

Specifications

Specifications

1μg/μL
CMV
20μg
−30 to −10°C
Transient and stable gene expression, cell imaging of Protein localization or trafficking in conjunction
High
pHTN HaloTag™ CMV-neo Flexi™ vector
10mM Tris HCl (pH 7.4), 1mM EDTA
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