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Promega™ GSH-Glo™ Glutathione Assay DFS Item

The GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or in various biological samples.

Manufacturer:  Promega™ V6911

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Catalog No. PRV6911



Includes: Reconstitution Buffer with esterase, Luciferin-NT, GSH-Glo™ Reaction Buffer, Glutathione S-Transferase, Glutathione (5mM), Luciferin Detection Reagent

Description

Description

  • Fast: Results in as little as 30 minutes
  • Simplified Method: Two-reagent-addition assay minimizes the number of assay steps compared to conventional GSH assays and is adapted easily to higher throughput applications; no deproteination step required!
  • Greater Sensitivity: luminescent method avoids inherent background fluorescence associated with other methods thereby providing excellent signal-to-background ratios
  • Stable Signal: Half-life greater than five hours
  • Use for: Measure GSH levels in cells or tissue extracts as an indicator of cell viability; Screen drugs and new chemical entities for modulation of GSH levels in cells, tissues or blood lysates

Specifications

Specifications

10mL
−30 to −10°C
1 x 10mL Reconstitution Buffer with Esterase, 1 x 100μL Luciferin-NT, 1 x 10mL GSH-Glo™ Reaction Buffer, 1 x 100μL Glutathione S-transferase, 1 x 100μL Glutathione 5mM, 1 x 1 each Luc
For detection and quantification of Glutathione in cells or in various biological samples
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