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Promega™ GoTaq™ qPCR Master Mix

Provides robust real-time qPCR with high levels of reliability, reproducibility and sensitivity

Manufacturer: promega™  A6001

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Catalog No. PRA6001

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Description & Specifications


Concentration 2X
For Use With (Application) Dye-Based Detection, qPCR and RT-qPCR
Includes 5 x 1mL GoTaq™ qPCR master mix, 2X, 1 x 100μL CXR reference dye, 2 x 13mL Nuclease free Water
No. of Reactions 200.0
Storage Requirements -20°C
Volume 200 x 50μL reactions

The system contains a new proprietary fluorescent DNA binding dye, BRYT™ GREEN, that exhibits greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR™ Green I. Thermal activation is achieved by incubating the assembled reaction at 95°C for two minutes. The proprietary polymerase/buffer formulation accommodates extended cycle numbers (45–50 cycles) and is compatible with thermal cycling programs that require extended activation (95°C for 10 minutes).

  • Pre-mixed, ready-to-use stabilized 2X formulation that includes all components for qPCR except sample DNA, primers and water to dilute the DNA standards
  • Detection of low copy targets
  • Enhanced stability for automated setup
  • Direct substitute for SYBR™ Green I products
  • The robust, reliable performance of GoTaq™ Hot Start Polymerase
  • The Promega PCR Performance Guarantee

Features - Benefits

Brighter Signal: Sensitive detection for earlier quantitation of low- and high-copy targets.
Enhanced Stability: Exceptional room-temperature setup makes it suitable for automation and high-throughput detection.
Versatile: Compatible with both fast and standard qPCR cycling methods.
Robust: High-efficiency, full-length cDNA synthesis in the presence of inhibitors.
Confidence: The Promega PCR Performance Guarantee.