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Promega™ GoTaq™ Probe qPCR Master Mix


Designed for quantitative PCR assays in hydrolysis probe detection format

Manufacturer: promega™  A6102

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Catalog No. PRA6102

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Description & Specifications

Specifications

Compatibility Both standard and fast instrument cycling programs
For Use With (Application) Sensitive sequence detection and quantification, gene expression analysis, detection of sequence variants, fluorescent detection, full length cDNA synthesis
Includes 10 x 1mL 2X GoTaq probe qPCR master mix (dTTP), 2 x 200μL 30μM CXR reference dye, 13mL nuclease free water
No. of Reactions 1000.0
Quantity 1000 x 20μL
Storage Requirements -30° to -10°C
Concentration 2X

  • Superior Performance: Sensitive detection on any real-time instrument
  • Enhanced Stability: Exceptional room-temperature setup makes it suitable for automation and high-throughput detection
  • Versatility: Compatible with both fast and standard cycling methods
  • Robustness: High-efficiency, full-length cDNA synthesis in the presence of inhibitors
  • Confidence: The Promega PCR Performance Guarantee

  • Stabilized 2X formulation that includes all components for qPCR (except template, primers and probe)
  • System does not contain reference dye, however separate carboxy-X-rhodamine (CXR) reference dye tube is included, that can be used for amplification reactions
  • Resistant to wide range of PCR inhibitors
  • Based on antibody-mediated hot-start chemistry, that allows reaction set up to be performed at room temperature
  • Facilitates rapid hot-start activation and processive enzymes, making it compatible with both standard and fast instrument cycling programs

Sensitive sequence detection and quantification, gene expression analysis, detection of sequence variants, fluorescent detection.