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Promega™ GoTaq™ Hot Start Polymerase: Green Master Mix, 2X


Combines the robust amplification and sensitivity of GoTaq DNA Polymerase with hot-start technology to deliver even greater specificity

Manufacturer: promega™  M5123

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Catalog No. PR-M5123

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Description & Specifications

Specifications

Activation Time 2 min.
Concentration 5U/μL
For Use With (Application) Hot-start PCR, complex genomic templates, T-vector cloning, two-step RT-PCR
Includes 2X Hot start green master mix (1 x 25mL), Nuclease free Water (1 x 25mL)
No. of Reactions 1000.0
Storage Requirements -20°C

  • Proprietary antibody bound to the polymerase blocks polymerase activity and permits room-temperture setup, but only requires a two-minute activation at 94°-95°C
  • GoTaq Flexi Reaction Buffers contain a compound that increases sample density and serves as both reaction buffer and gel-loading solution, saving time in post-PCR anaylsis
  • Green GoTaq Flexi Buffer contains two dyes that separate during electrophoresis to facilitate direct-to-gel analysis of PCR products
  • Enzyme exhibits 5' to 3' exonuclease activity

Hot-start PCR, complex genomic templates, T-vector cloning, two-step RT-PCR