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Promega™ GoTaq™ Hot Start Polymerase: Colorless Master Mix, 2X


Combines the robust amplification and sensitivity of GoTaq DNA Polymerase with hot-start technology to deliver even greater specificity

Manufacturer: promega™  M5132

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Catalog No. PR-M5132

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Description & Specifications

Specifications

Activation Time 2 min.
Concentration 5U/μL
For Use With (Application) Hot-start PCR, complex genomic templates, T-vector cloning, two-step RT-PCR
Includes 2X Hot start colorless master mix (2 x 1250μL), Nuclease free Water (2 x 1250μL)
No. of Reactions 100.0
Storage Requirements -20°C

  • Proprietary antibody bound to the polymerase blocks polymerase activity and permits room-temperture setup, but only requires a two-minute activation at 94°-95°C
  • GoTaq Flexi Reaction Buffers contain a compound that increases sample density and serves as both reaction buffer and gel-loading solution, saving time in post-PCR anaylsis
  • Colorless GoTaq Flexi Buffer is used when direct fluorescence or absorbance readings are required without prior purification of the DNA from the amplification reaction

Hot-start PCR, complex genomic templates, T-vector cloning, two-step RT-PCR