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Promega™ GoTaq™ Hot Start Polymerase: Colorless Master Mix, 2X DFS Item

GoTaq™ Hot Start Master Mixes are all-in-one master mixes (just add template and primers) that contain the high-performance GoTaq™ DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 94-95°C for two

Manufacturer:  Promega™ M5133

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Catalog No. PR-M5133



Description

Description

  • Proprietary antibody bound to the polymerase blocks polymerase activity and permits room-temperture setup, but only requires a two-minute activation at 94°-95°C
  • GoTaq Flexi Reaction Buffers contain a compound that increases sample density and serves as both reaction buffer and gel-loading solution, saving time in post-PCR anaylsis
  • Colorless GoTaq Flexi Buffer is used when direct fluorescence or absorbance readings are required without prior purification of the DNA from the amplification reaction

Hot-start PCR, complex genomic templates, T-vector cloning, two-step RT-PCR

Specifications

Specifications

2 min.
2X Hot start green master mix (1 x 25mL), Nuclease free Water (1 x 25mL)
-20°C
Hot-start PCR, complex genomic templates, T-vector cloning, two-step RT-PCR
1000.0
5U/μL
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