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Promega GoTaq™ Hot Start Polymerase: 5X DFS Item

GoTaq™ Hot Start Polymerase contains the high-performance GoTaq™ DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 94-95°C for two minutes.

Manufacturer:  Promega M5001

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Catalog No. PRM5001

Includes: GoTaq Hot Start Polymerase is supplied with 5X Colorless GoTaq Flexi Buffer, 5X Green GoTaq Flexi Buffer, and 25mM MgCl2.



  • Robust PCR with Convenient Room-Temperature Setup
  • Minimize nonspecific amplification and primer-dimers
  • Optimize reaction conditions with supplied Flexi Reaction Buffer and MgCl2 solution
  • Direct-to-gel convenience
  • Supplied at a concentration of 5u/µL

U.S. Patent No. 6,242,235, Australian Patent No. 761757, Canadian Patent No. 2,335,153.
Licensed under U.S. Patent Nos. 5,338,671 and 5,587,287 and corresponding patents in other countries.



2 min.
5X Hot start Polymerase (1 x 100U), colorless flexi Buffer (1 x 1mL), 5X green flexi Buffer (1 x 1mL), MgCl2 (1 x 750μL)
−30 to −10°C
Hot-start PCR, complex genomic templates, T-vector cloning, two-step RT-PCR