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Promega™ GoTaq™ Flexi DNA Polymerase

Combines robust amplification, streamlined downstream analysis, and the flexibility to optimize Mg2+ concentration

$36.00 - $1,379.00

Specifications

Concentration 5U/μL
Format Liquid
For Use With (Application) Samples can be loaded directly onto a gel after amplification
Includes GoTaq Flexi DNA Polymerase, 5X Colorless GoTaq Flexi buffer (Used when direct fluorescence or absorbance readings are required without prior purification of the DNA from the amplification reaction), 5X Green GoTaq Flexi buffer, 25mM MgCl2
Storage Requirements -20°C
View More Specs
Includes:  GoTaq Flexi DNA Polymerase, 5X Colorless GoTaq Flexi Buffer (Used when direct fluorescence or absorbance readings are required without prior purification of the DNA from the amplification reaction), 5X Green GoTaq Flexi Buffer (Contains two dyes that separate during electrophoresis to facilitate direct-to-gel analysis of PCR products), 25mM MgCl2
Products
Catalog Number Mfr. No. Product Name Quantity Price Quantity    

PR-M8291

 
promega™
M8291
DNA Polymerase 100U Each for $36.00

PRM8296

 
promega™
M8296
DNA Polymerase 2500U Each for $710.00

PR-M8295

 
promega™
M8295
DNA Polymerase 500U Each for $161.00

PR-M8297

 
promega™
M8297
DNA Polymerase 5000U Each for $1,035.00

PRM8298

 
promega™
M8298
DNA Polymerase 10000U Each for $1,379.00
Description & Specifications

Specifications

Concentration 5U/μL
Format Liquid
For Use With (Application) Samples can be loaded directly onto a gel after amplification
Includes GoTaq Flexi DNA Polymerase, 5X Colorless GoTaq Flexi buffer (Used when direct fluorescence or absorbance readings are required without prior purification of the DNA from the amplification reaction), 5X Green GoTaq Flexi buffer, 25mM MgCl2
Storage Requirements -20°C

  • Offers robust amplification equal to—and in some cases superior to—conventional Taq DNA polymerase
  • Suitable for PCR, two-step RT-PCR, and T-vector cloning
  • Utilizes a separate MgCl2 solution to allow for optimization of magnesium concentration
  • Samples can be loaded directly onto a gel after amplification
  • Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10μg activated calf thymus DNA and 0.1mg/mL BSA in a final volume of 50μL.

The purchase of this product conveys to the buyer the limited, nonexclusive, nontransferable right (without the right to resell, repackage, or further sublicense) under U.S. Published Patent Appln. 20020150900 and U.S. Pat. Nos. 5,432,272, 6,617,106 and 6,140,496 to use the product. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include or carry any right or license to sell this product. For information on purchasing a license for other uses, please contact Promega Corporation, Licensing, 2800 Woods Hollow Road, Madison, WI 53711, or EraGen Biosciences, Corporate Licensing, 918 Deming Way, Suite 201, Madison, WI 53717. Phone (608) 662-9000; Fax (608) 662-9003.
This product is designed and sold for use in the multiplex PCR process covered by U.S. Pat. No. 5,582,989 and Canadian Pat. No. 1,339,731. A limited license has been granted under the patent to use only this amount of the product to practice the multiplex PCR process and is conveyed to the purchaser by the purchase of this product.
Patents for the foundational PCR process, European Pat. Nos. 201,184 and 200,362, expired on March 28, 2006. In the U.S., the patents covering the foundational PCR process expired on March 29, 2005.