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Promega™ GoTaq™ DNA Polymerase DFS Item

Taq DNA Polymerase. Provided with Green and Colorless Reaction Buffers. Green Buffer allows direct gel analysis after amplification. Enhanced performance over standard Taq DNA Polymerase.

Manufacturer:  Promega™ M3001

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Catalog No. PR-M3001



Description

Description

  • Offer robust amplification equal to—and in some cases superior to—conventional Taq DNA polymerase
  • Suitable for PCR, two-step RT-PCR, and T-vector cloning
  • Samples can be loaded directly onto a gel after amplification
  • Compatible with PCR enhancers such as betaine and DMSO
  • Premixed, ready-to-use solutions contain GoTaq DNA polymerase, dNTPs, MgCl2, and reaction buffers in a single tube
  • Utilize an optimized enzyme and buffer formulation that offers amplification typically better than standard Taq DNA polymerases
  • Can be directly substituted into existing PCR protocols without the need to change cycling parameters
  • GoTaq Green Master Mix contains a blue dye and a yellow dye that allow the monitoring of progress during electrophoresis
  • Utilize a separate MgCl2 solution to allow for optimization of magnesium concentration
  • Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10μg activated calf thymus DNA and 0.1mg/mL BSA in a final volume of 50μL

Specifications

Specifications

PCR, two-step RT-PCR and T-vector cloning
>90% Pure
−30 to −10°C
GoTaq™ DNA Polymerase, 1mL 5X green GoTaq™ reaction Buffer and 1mL 5X colorless GoTaq™ reaction Buffer, MgCl2 at a concentration of 7.5mM, nuclease free water
100U
5U/μL
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