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Promega™ GeneClip™ U1 Hairpin Cloning Systems


Designed for fast and easy cloning of human hairpin target sequences to allow expression of siRNA target sequences in human cells

Manufacturer: promega™  C8790

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Catalog No. PR-C8790

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Description & Specifications

Specifications

For Use With (Application) Fast and easy cloning of Human hairpin target sequences to allow expression of siRNA target sequences in Human cells
Format Liquid
Includes 200μL 2X rapid Ligation Buffer, 1.25mL Nuclease-free Water, 1mL Oligo annealing Buffer, 1.2μg pGeneClip hMGFP vector, 100U T4 DNA Ligase
pH 7.4
Quantity 20 Reactions

Use pGeneClip™ vectors containing the human U1 promotor, which allows transcription of the hairpin target sequences and generation of hairpin siRNA in vivo.

  • Utilize predigested vectors to eliminate time-consuming vector preparation
  • Allow easier identification of desired clones using a Pst I digestion

  • Available with a choice of vectors
    • The hMGFP System enables easy determination of transfection efficiency
    • The other systems offer antibiotic marker options for selecting stably transfected eukaryotic cells so that experimental results do not depend on transfection efficiency