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Promega™ Gel Shift Assay Systems

Simple, rapid systems for detecting DNA-binding proteins

$479.36 - $595.99

Specifications

Quantity 100 Reactions
For Use With (Application) Used to study sequence specific DNA binding Proteins such as transcription facto rs
Includes HeLaScribe™ nuclear extract, 40μL Gel shift assay grade, T4 Polynucleotide Kinase, 100U Kinase Buffer (10X), 100μL Gel shift Binding Buffer (5X), 200μL, AP2 and SP1 Consensus Oligonucleotides, 35pmol each.
Storage Requirements HeLa nuclear extract at -70°C and other components at -20°C
View More Specs
Includes:  HeLaScribe Nuclear Extract, Gel Shift Assay Grade, 40μL (also available separately); T4 polynucleotide kinase, 100U; Kinase 10X buffer, 100μL; Gel shift binding 5X buffer, 200μL; AP2 extract, 20μL; Consensus oligonucleotides (see table), 35pmol each
Products
Catalog Number Mfr. No. Includes Product Type Price Quantity    

PR-E3050

 
promega corportaion
E3050
HeLaScribe™ nuclear extract, 40μL Gel shift assay grade, T4 Polynucleotide Kinase, 100U Kinase Buffer (10X), 100μL Gel shift Binding Buffer (5X), 200μL, AP2 and SP1 Consensus Oligonucleotides, 35pmol each. Gel Shift Assay Core System Each for $479.36

PR-E3300

 
promega™
E3300
HeLaScribe™ nuclear extract, 40μL Gel shift assay grade, T4 Polynucleotide Kinase, 100U Kinase Buffer (10X), 100μL Gel shift Binding Buffer (5X), 200μL, AP2, SP1, AP1, OCT1, CREB, NF-κB and TFIID Consensus Oligonucleotide, 35pmol each Gel Shift Assay Complete System Each for $595.99
Description & Specifications

Specifications

Quantity 100 Reactions
For Use With (Application) Used to study sequence specific DNA binding Proteins such as transcription facto rs
Includes HeLaScribe™ nuclear extract, 40μL Gel shift assay grade, T4 Polynucleotide Kinase, 100U Kinase Buffer (10X), 100μL Gel shift Binding Buffer (5X), 200μL, AP2 and SP1 Consensus Oligonucleotides, 35pmol each.
Storage Requirements HeLa nuclear extract at -70°C and other components at -20°C

The gel shift assay is based on the observation that complexes of protein and DNA migrate through a nondenaturing polyacrylamide gel more slowly than free DNA fragments or double-stranded oligonucleotides. The specificity of a DNA-binding protein for the suspected binding site is established by competition experiments using a 32P end-labeled DNA fragment containing the suspected binding site and unlabeled DNA fragments or oligonucleotides containing a binding site for the protein of interest or other unrelated DNA sequences.

  • The Core System contains an E. coli extract containing cloned AP2 protein and an AP2 consensus oligo, making it a reliable system for gaining experience with gel shift assays, due to the strong gel shift produced by AP2
  • The Complete System contains five additional double-stranded oligonucleotides that represent consensus sequences to characterized binding sites
  • Provided oligonucleotides can be 5« end-labeled and used as protein-specific probes or used as unlabeled oligonucleotides in competition assays