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Promega™ Gel Shift Assay Systems

Simple, rapid systems for detecting DNA-binding proteins

Manufacturer: promega™  E3300

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Catalog No. PR-E3300

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Description & Specifications


Product Type Gel Shift Assay Complete System
For Use With (Application) Used to study sequence specific DNA binding Proteins such as transcription facto rs
Quantity 100 Reactions
Includes HeLaScribe™ nuclear extract, 40μL Gel shift assay grade, T4 Polynucleotide Kinase, 100U Kinase Buffer (10X), 100μL Gel shift Binding Buffer (5X), 200μL, AP2, SP1, AP1, OCT1, CREB, NF-κB and TFIID Consensus Oligonucleotide, 35pmol each
Storage Requirements HeLa nuclear extract at -70°C and other components at -20°C

The gel shift assay is based on the observation that complexes of protein and DNA migrate through a nondenaturing polyacrylamide gel more slowly than free DNA fragments or double-stranded oligonucleotides. The specificity of a DNA-binding protein for the suspected binding site is established by competition experiments using a 32P end-labeled DNA fragment containing the suspected binding site and unlabeled DNA fragments or oligonucleotides containing a binding site for the protein of interest or other unrelated DNA sequences.

  • The Core System contains an E. coli extract containing cloned AP2 protein and an AP2 consensus oligo, making it a reliable system for gaining experience with gel shift assays, due to the strong gel shift produced by AP2
  • The Complete System contains five additional double-stranded oligonucleotides that represent consensus sequences to characterized binding sites
  • Provided oligonucleotides can be 5« end-labeled and used as protein-specific probes or used as unlabeled oligonucleotides in competition assays